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| Bellec, Laure; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Durand, Lucile; Gayet, Nicolas; Zeppilli, Daniela. |
| Deep-sea hydrothermal vent meiofauna have been the focus of recent research and the discovery of an abundant well-adapted free-living marine nematode on the Mid-Atlantic Ridge offers new perspectives on adaptations to the vent environment. Indeed, knowledge concerning biological interactions of microbes and meiofauna in marine extreme environments is scarce, especially for nematodes. In this study, we used microscopic observations [fluorescence in situ hybridization (FISH) and scanning electron microscopy (SEM)] and metabarcoding of 16S rRNA to characterize the bacterial community of the nematode species Oncholaimus dyvae, an overlooked but ecologically important vent organism. Detection of bacteria in the buccal cavity and on the cuticle (SEM) and... |
| Tipo: Text |
Palavras-chave: Sulfur-oxidizing bacteria; Lucky Strike vent field; Meiofauna; Nematode; Endosymbiont. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00457/56846/58676.pdf |
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| Bellec, Laure; Cambon Bonavita, Marie-anne; Hourdez, Stéphane; Jebbar, Mohamed; Tasiemski, Aurélie; Durand, Lucile; Gayet, Nicolas; Zeppilli, Daniela. |
| Prokaryotes and free-living nematodes are both very abundant and co-occur in marine environments, but little is known about their possible association. Our objective was to characterize the microbiome of a neglected but ecologically important group of free-living benthic nematodes of the Oncholaimidae family. We used a multi-approach study based on microscopic observations (Scanning Electron Microscopy and Fluorescence In Situ Hybridization) coupled with an assessment of molecular diversity using metabarcoding based on the 16S rRNA gene. All investigated free-living marine nematode specimens harboured distinct microbial communities (from the surrounding water and sediment and through the seasons) with ectosymbiosis seemed more abundant during summer.... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00494/60614/64098.pdf |
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| Bellec, Laure; Clerissi, Camille; Edern, Roseline; Foulon, Elodie; Simon, Nathalie; Grimsley, Nigel; Desdevises, Yves. |
| Background: Numerous studies have investigated cospeciation (or cophylogeny) in various host-symbiont systems, and different patterns were inferred, from strict cospeciation where symbiont phylogeny mirrors host phylogeny, to complete absence of correspondence between trees. The degree of cospeciation is generally linked to the level of host specificity in the symbiont species and the opportunity they have to switch hosts. In this study, we investigated cophylogeny for the first time in a microalgae-virus association in the open sea, where symbionts are believed to be highly host-specific but have wide opportunities to switch hosts. We studied prasinovirus-Mamiellales associations using 51 different viral strains infecting 22 host strains, selected from... |
| Tipo: Text |
Palavras-chave: Cophylogeny; Prasinovirus; Phycodnaviridae; Mamiellale; Chlorophyta; Specificity. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00374/48497/48827.pdf |
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| Zeppilli, Daniela; Bellec, Laure; Cambon-bonavita, Marie-anne; Decraemer, Wilfrida; Fontaneto, Diego; Fuchs, Sandra; Gayet, Nicolas; Mandon, Perrine; Michel, Loic; Portail, Marie; Smol, Nic; Sørensen, Martin V.; Vanreusel, Ann; Sarrazin, Jozee. |
| Background Nematodes are an important component of deep-sea hydrothermal vent communities, but only few nematode species are able to cope to the harsh conditions of the most active vent sites. The genus Oncholaimus is known to tolerate extreme geothermal conditions and high sulphide concentrations in shallow water hydrothermal vents, but it was only occasionally reported in deep-sea vents. In this study, we performed morphological, genetic and ecological investigations (including feeding strategies) on an abundant species of Oncholaimus recently discovered at Lucky strike vent field on the Mid-Atlantic Ridge at 1700 m water depth. Results We described this species as Oncholaimus dyvae sp. nov.. This new species differs from all other members of the genus... |
| Tipo: Text |
Palavras-chave: Oncholaimus dyvae sp. nov.; Hydrothermal vents; Deep sea; Carbon and nitrogen isotopic ratios. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00508/61985/66097.pdf |
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| Bellec, Laure; Cambon-bonavita, Marie-anne; Durand, Lucile; Aube, Johanne; Gayet, Nicolas; Sandulli, Roberto; Brandily, Christophe; Zeppilli, Daniela. |
| Shallow-water hydrothermal vents are widespread, especially in the Mediterranean Sea, owing to the active volcanism of the area. Apart free microbial communities’ investigations, few biological studies have been leaded yet. Investigations of microbial communities associated with Nematoda, an ecologically important group in sediments, can help to improve our overall understanding of these ecosystems. We used a multidisciplinary-approach, based on microscopic observations (scanning electron microscopy: SEM and Fluorescence In Situ Hybridization: FISH) coupled with a molecular diversity analysis using metabarcoding, based on the 16S rRNA gene (V3-V4 region), to characterize the bacterial community of a free-living marine nematode and its environment, the... |
| Tipo: Text |
Palavras-chave: Nematode; Shallow-water hydrothermal vent; Sulfur-oxidizing bacteria; Iron cycle; Zetaproteobacteria. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75544/76449.pdf |
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| Bellec, Laure; Louboutin, Lenaig; Cabon, Joelle; Castric, Jeanne; Cozien, Joelle; Thiery, Richard; Morin, Thierry. |
| Infectious hematopoietic necrosis virus (IHNV) is among the most important pathogens affecting the salmonid industry. Here, we investigated the molecular evolution and circulation of isolates from 11 countries or regions all over the world, with a special focus on the epidemiological situation in France. The phylogeography, time to the most recent common ancestor (TMRCA) and nucleotide substitution rate were studied using 118 full-length glycoprotein gene sequences isolated from 9 countries (5 genogroups) over a period of 47 years. The TMRCA dates back to 1943, with the L genogroup identified as the likely root (67 %), which is consistent with the first report of this pathogen in the USA. A Bayesian inference approach was applied to the partial... |
| Tipo: Text |
Palavras-chave: Infectious hematopoietic necrosis virus; Evolutionary dynamics; Dates of divergence; Phylogeography; Molecular epidemiology. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00399/51015/51832.pdf |
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